Alkalicella caledoniensis gen. nov., sp. nov., a novel alkaliphilic anaerobic bacterium isolated from ‘La Crouen’ alkaline thermal spring, New Caledonia

A novel anaerobic, alkaliphilic, mesophilic, Gram-stain-positive, endospore-forming bacterium was isolated from an alkaline thermal spring (42 °C, pH 9.0) in New Caledonia. This bacterium, designated strain LB2 T , grew at 25–50 °C (optimum, 37 °C) and 8.2–10.8 9.5). Added NaCl not required for growth 0–1 %) but tolerated up to 7 %. Strain utilized a limited range of substrates, such as peptone, pyruvate, yeast extract xylose. End products detected pyruvate fermentation were acetate formate. Both ferric citrate thiosulfate used electron acceptors. Elemental sulphur, nitrate, nitrite, fumarate, sulphate, sulfite DMSO terminal The two major cellular fatty acids iso-C 15 : 0 C 16 . genome consists circular chromosome (3.7 Mb) containing 3626 predicted protein-encoding genes with G+C content 36.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that isolate is member family Proteinivoraceae order Clostridiales within phylum Firmicutes most closely related thermophilic Anaerobranca gottschalkii LBS3 (93.2 % identity). Genome-based average nucleotide identity digital DNA–DNA hybridization A. showed respective values 70.8 13.4 Based phylogenetic, genomic, chemotaxonomic physiological properties, proposed represent first species genus, which name Alkalicella caledoniensis gen. nov., sp. nov. (type =DSM 100588 =JCM 30958 ).